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Hereford and Red Angus Heifers Recruited for Genomics Research

The University of Missouri is recruiting 2,500 Hereford heifers and 2,500 Red Angus heifers to participate in a heifer puberty and fertility genomic research project. Heifers should be registered Hereford, registered Red Angus, or commercial Hereford or Red Angus. Hereford x Red Angus crossbred heifers targeted for the Premium Red Baldy Program would also be a good fit for the research project. Producers must be willing to work with a trained veterinarian to collect the following data: ReproductiveTract Scores collected at a pre-breeding exam 30 to 45 days prior to the start of the breeding season. PelvicMeasurements (height and width) collected at the same pre-breeding exam 30 to 45 days prior to the start of the breeding season. Pregnancy Determination Using Ultrasound reporting fetal age in days. Ultrasound will need to occur no later than 90 days after the start of the breeding season. In addition, heifers must have known birth dates and have weights recorded eithe

CIC 2019: Unraveling the Secrets of the Rumen

Tim McAllister
AAFC Lethridge Research & Development Centre

Microbes are part of the natural world, they occupy all parts of the world from deep sea vents, skin, digestive tract, and our food.

The rumen is one of the most microbe rich environments in the world. We use fistulated animals to have a window into the rumen microbiome. Rather than looking at the bacteria that come out of the digestive tract, we can directly sample the rumen. The microbes in cattle rumens can even digest cotton shirts!

In cattle, the majority of digestion happens in the rumen by the microbes. So, most of the time when we are feeding our cows, we are really feeding the microbes.

Cows eat 2 to 20 kg of dry matter, 20 to 80 liters of water, and 50 to 150 liters of saliva. The microbes produce 2 to 6 kilograms of volatile fatty acids (VFA). The rumen also produces 0.75 to 2 kilograms of bacteria that go into the digestive system and can be digested.

In a roughage diet, 65% of VFA is acetate. Cattle on high concentrate diets produce more propionate; the propionate provides more energy to the cattle, which is part of the reason cattle on concentrate grow faster.

It is hard to culture bacteria that live in the rumen. However, we can extract DNA and RNA from a sample from the digestive system. We can then sequence this DNA. When we do this, we realize that we can only culture 5% of the microbes that live in the rumen.

In every milliliter of rumen fluid there are 10 billion to 100 billion bacterial cells. There are also anaerobic fungi, ciliate protozoa, and methanogenic archaea.

Protozoa are predators. They eat other microbes in the rumen. The number and diversity of protozoa are higher in a forage diet. From an energetic perspective, the protozoa eating bacteria are robbing from the cattle.

Fungi have the most complex life cycle of microbes in the rumen. One stage swim around and find a place to latch onto. They then produce root-like structure that borrow into plant fibers. This is like the roots of a tree breaking up concrete. The bacteria can't do this, so the fungi create more surfaces for the bacteria to attached to.

Microbia get into the rumen through maternal transfer, feces, feed transfer, housing, water, and products. The colonization takes 2 to 20 days after birth. Ther first are aerobic microbes. They help create an anaerobic environment. The final microbes to be established are the predators.

From the 1st, to 2nd, to 3rd weeks after birth the rumen microbiome becomes more complex. As the community of microbes becomes more diverse, the types of digestive functions that they can complete increase. Once the rumen of an animal evolves to digest a diet (say roughage), those microbes remain in the rumen at low levels for the rest of the animals life. This means an animal can switch from a forage diet to a concentrate diet and back to a forage diet because the microbes needed to digest that diet are still in the rumen.

The rate of carbohydrate digestion depends on how complex the carbohydrate is. Simple sugars are digested rapidly. Cellulose (fiber) takes as long as 24 hours.

There are primary and secondary colonizers. They work together to create a bio-film around plant particles.

Microbes hate fats. They also cannot digest lignins in the anaerobic environment of the rumen. Seeds are coated in lipids and lignins, which is why we process grains before feeding to cattle.

There are differences in how well animals chew their feed. An animal that chews their feed more will allow the microbes to more efficiently digest the feed.

Ammonia fiber expansion (AFEX) is a chemical treatment of low quality feed. This allows wheat straw to be as high quality of a feed as alfalfa.

The number one determinant of the microbes in the rumen is the type of feed being feed. Animals like deer that are browsers that eat a variety of food have a more diverse rumen microbial community.

Scientific Reportsvolume 7, Article number: 1276 (2017) 
If we transfer the rumen content of bison into canulated cattle, what happens? They replaced 70% of the cattle rumen contents with bison rumen contents. After 27 days, the cattle rumens evolved to be more like the original cattle rumens, but they were also different now.

What strategies reduce methane emissions?

  • improving forage quality
  • grazing management
  • feed processing
  • precision (balanced) feeding and feed analysis


One feed yard was losing $10,000 worth of nutrients because they were not processing their feed enough and there was undigested starch in grain kernels in the feces.

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